Testing for Macromolecules

Purpose: To determine which chemical indicators that will indicate the armorial bearing of glucose, starch, lipid, and protein in various tastes. Methods and Materials: For materials, disport come to to p.18 in Biology 11 (replace Sudan IV with filter penning) For procedures, disport refer to p.18-19 (except get around C) For Part C in Inv. 1A, please read the following procedures: 1.Label render 1-6 on the filter paper 2.Add one drop of each sample corresponding to their play indicated on the filter paper (for the number of each sample, please refer to step 4 in Part A on p.18) 3.Leave the filter paper to alter for approximately 5-10 proceedings 4.Record observations Results: Table 1: Reactions of sample substances to indicators SAMPLEBenedicts final result + heatIodine solutionFilter paperBiuret reagent 1.protein solutionPurpleNo agitateDried upPurple 2.vege evade oilNo motleyNo transportPaper remained translucentNo change 3.glucose solutionOrangeNo changeDried upN o change 4.sucrose solutionNo changeNo changeDried upNo change 5.starch solutionNo changeDark blue-blackDried upNo change 6.distilled waterNo change (Clear blue)No change (Clear yellow)Dried upNo change Investigation 1A: Testing for Macromolecules Discussion: By observing the table above, we could see that Benedicts test was for reduction sugars, iodine test was for the front of starch, filter paper was for the bearing of fatty acids, and the Biuret test was for amino conclaves present in proteins.

Benedicts solution was used to test for the presence of simple sugars, such as glucose (monosaccharide). When heated, the sol ution mixed with monosaccharides produced a ! reddish-orange colour. This was because Benedicts solution is composed of atomic number 11 citrate, sodium carbonate, and cupric sulfate pentahydrate. When solution is heated, an oxidation-reduction reply occurs: cupric ion (Cu+2) oxidizes into a cuprous ion (C+) and precipitates into cuprous oxide (Cu2O) because Benedicts solution loses an type O (Cu+2 ---> Cu+ forming Cu2O). Basically, it is a reaction between the aldehyde grouping (-CHO) of the... If you want to get a full essay, order it on our website: BestEssayCheap.com

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